This purpose of this study was to conduct a survey of anthrax (Bacillus anthracis) in the 19 buffer villages that surround Ujung Kulon National Park in Java, Indonesia. Soil specimens were collected from water buffalo terminals in each village, and when possible specifically from areas with a history of sudden water buffalo death. Twenty-three samples were collected and analyzed in a microbiology laboratory at the Institut Pertanian Bogor. Colony description, tryptic soy agar, brain heart infusion broth, and microscopic analysis were used to support a suspected diagnosis of B.anthracis. Six samples were suspected and brought to Balai Besar Penelitian Veteriner Bogor for PCR analysis. All samples were negative although we are not completely confident in this report and will pursue one further step for confirmation.